User manual LEICA LMD6500 BROCHURE

[. . . ] This makes specimen collection by gravity a contamination-free procedure. Transfer dissectates of any size or shape Gravity is the ideal tool to collect dissectates of any size or shape ­ round and compact, or long and thin ­ it simply falls into the collection device. This ensures the complete recovery of the sample without the loss of tissue due to additional cut lines. Collect directly into reaction buffer Collection by gravity is the fastest way to progress from tissue section to reaction buffer. [. . . ] The image remains fixed and only the laser beam moves ­ just the way our eyes are used to. Benefits: Highest possible precision Maximal speed Real-time laser cutting Convenient movie documentation Maintenance free Step 1: Define region of interest Step 2: Laser beam steered by optics along the cut line Step 3: Specimen collection by gravity * Patented EP 1276586 B1, US 7035004 B2, JP 3996773 B2, TW 486566 B 5 6 Leading-edge Laser Technology The laser is the core part of a laser microdissection system. The combination of these features within a LMD system was realized by Leica Microsystems for the first time. Adapt the laser to the specimen The Leica LMD7000 allows the control of laser pulse energy and frequency. Adjust the laser according to the specimen and make narrow, powerful, and fast cuts within a single system. Leica Microsystems' LMD systems provide the flexibility to align the UV offset with respect to the focus of the visible light for the most precise cutting performance, for thick or thin samples, and ablation. Control the thickness of the cutting line The aperture control enables the adjustment of the width of the cutting line. Combined with the high repetition rate, the narrowest cuts are possible. The laser aperture settings can be stored individually for each objective. LMD Laser Systems Leica LMD7000 120 J Single pulse to 5000 Hz Yes 349 nm Yes Leica LMD6500 50 J 80 Hz No 355 nm Yes Benefits: Control the pulse energy and frequency (for Leica LMD7000) Adjust the width of the cutting line Control the UV offset Maintenance free solid state laser Longlife, solid state laser Leica Microsystems uses diode-pumped, solid state lasers. These state-of-the-art lasers are maintenance free and reliable, ensuring long lifetimes. Choose the laser according to your needs: the Leica LMD6500 offers a pulse energy of 50 J and a fixed repetition rate, whereas the Leica LMD7000 provides a pulse energy of up to 120 J and the unique flexibility to adjust power and frequency. Please visit www. leica-microsystems. com/lmd for more images and movies, application letters, etc. Max. pulse energy Repetition rate Adjustable repetition rate Wavelength Laser aperture control Fig. 2: Primary cell culture from pancreatic adenocarcinoma (PDAC) stained with hematoxylin, objective 10x (before cutting, after cutting and inspection). Courtesy of Axaron Bioscience AG, Heidelberg, Germany 7 System Integration Benefits: Fully automated DIC Motorized fluorescence axis with 5- or 8-position filter cube turret Fluorescence intensity manager (FIM) Motorized Excitation Manager and fast Internal Filter Wheel (IFW) Convenient Leica SmartTouch touchscreen for controlling the automated modules All components of the Leica LMD systems are fully integrated and act in concert, building a winning team greater than the sum of its components. High-end Leica DM6000 B research microscope The foundation of every Leica LMD system is the high-end Leica DM6000 B upright research microscope, which is suitable for all types of life science research. Excellent optical performance, intelligent automation, and user-friendly operation make this fully automated microscope the perfect tool for microscopic research. Specially designed optics for laser microdissection Leica Microsystems' dedicated LMD objectives feature the highest possible UV transmission and outstanding imaging performance ­ the Leica SmartCut series. The unique high magnification 150x dry objective enables the dissection of even the smallest areas without using oil. User-friendly software Leica Microsystems' LMD software is very easy to use, yet powerful without being complicated. [. . . ] Our strong focus on understanding scientific applications puts Leica Microsystems' customers at the leading edge of science. The statement by Ernst Leitz in 1907, "with the user, for the user, " describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life. Active worldwide Australia: Austria: Belgium: Canada: Denmark: France: Germany: Italy: Japan: Korea: Netherlands: People's Rep. of China: Portugal: Singapore Spain: Sweden: Switzerland: United Kingdom: USA: Barcelona Kista Heerbrugg Milton Keynes Bannockburn/lllinois North Ryde Vienna Groot Bijgaarden Richmond Hill/Ontario Herlev Nanterre Cedex Wetzlar Milan Tokyo Seoul Rijswijk Hong Kong Lisbon Tel. [. . . ]