User manual LEICA CRYOFECT

[. . . ] The system's individual components can be used in a variety of embedding procedures to perfectly embed and properly orient almost any type of specimen. The process is comfortably performed inside the cryostat using stainless steel well bars, chucks, and freezing blocks. [. . . ] The process is comfortably performed inside the cryostat using stainless steel well bars, chucks, and freezing blocks. Since the Precision Cryoembedding System's components are stored at cold temperatures, most specimens freeze in 20 to 60 seconds, depending upon their size and the selected freezing technique. And, your well-oriented, flat specimens will be conserved during the trimming process!What else could you ask for? Cryoembedding components in the Leica CM1850 cryochamber The Precision Cryoembedding System consists of: · 3 well bars in 3 sizes (18, 24 and 30 mm) · 6 small stages (chucks) · 4 large stages (chucks) · 4 over-stage heat extractors · 1 storage bin for stages · 16 dispensing slides · 1 cutting board/freezing griddle · 1 elevated heat extractor · 1 pair of angled embedding forceps and accessories Precision cryoembedding accessories Face Down Cryoembedding Perfect, Flat Orientation Any specimen, whether it is single or multiple pieces; large or minute; solid or liquid, can be embedded flat and in a single plane. Technique 1. Precisely orient the specimen(s), face down, on a thin plastic dispensing slide. Touch the edge of a specimen to the cold base of the embedding well and gently withdraw the dispensing slide while positioning the specimen. Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition. The companies of the Leica Microsystems Group operate internationally in four business segments, where we rank with the market leaders. · Microscopy Systems Leica Microsystems ­ an international company with a strong network of customer services Australia: Austria: Canada: Denmark: France: Germany: Italy: Japan: Korea: Netherlands: People's Rep. of China: Portugal: Singapore Spain: Sweden: Switzerland: United Kingdom: USA: Gladesville Vienna Richmond Hill/Ontario Herlev Rueil-Malmaison Bensheim Milan Tokyo Seoul Rijswijk Hong Kong Lisbon Barcelona Sollentuna Glattbrugg Milton Keynes Bannockburn/lllinois Tel. +1 847 405 0123 Fax +61 2 9817 8358 Fax +43 1 486 80 50 30 Fax +1 905 762 8937 Fax +45 4454 0111 Fax +33 1 473 285 86 Fax +49 6251 136 155 Fax +39 0257 40 3273 Fax +81 3 5435 9615 Fax +82 2 514 65 48 Fax +31 70 4132 109 Fax +852 2564 4163 Fax +351 21 385 4668 Fax +65 6773 0628 Fax +34 93 494 95 32 Fax +46 8 625 45 10 Fax +41 1 809 34 44 Fax +44 1908 609 992 Fax +1 847 405 0164 Our expertise in microscopy is the basis for all our solutions for visualization, measurement and analysis of microstructures in life sciences and industry. With confocal laser technology and image analysis systems, we provide threedimensional viewing facilities and offer new solutions for cytogenetics, pathology and materials sciences. · Specimen Preparation · Medical Equipment Innovative technologies in our surgical microscopes offer new therapeutic approaches in microsurgery. @ www. histo-solutions. com Leica Microsystems Nussloch GmbH Heidelberger Strasse 17-19 D-69226 Nussloch Telephone: (06224) 143-0 Fax: (06224) 143 200 e-mail: histo_info@leica-microsystems. com www. leica-microsystems. com 09/2004 and representatives of Leica Microsystems in more than 100 countries. · Semiconductor Equipment Our automated, leading-edge measurement and inspection systems and our E-beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world. ©Leica Microsystems Nussloch GmbH, Heidelberger Str. (06224) 143-0, Fax (06224) 143-200 We provide comprehensive systems and services for clinical histo- and cytopathology applications, biomedical research and industrial quality assurance. [. . . ] +1 847 405 0123 Fax +61 2 9817 8358 Fax +43 1 486 80 50 30 Fax +1 905 762 8937 Fax +45 4454 0111 Fax +33 1 473 285 86 Fax +49 6251 136 155 Fax +39 0257 40 3273 Fax +81 3 5435 9615 Fax +82 2 514 65 48 Fax +31 70 4132 109 Fax +852 2564 4163 Fax +351 21 385 4668 Fax +65 6773 0628 Fax +34 93 494 95 32 Fax +46 8 625 45 10 Fax +41 1 809 34 44 Fax +44 1908 609 992 Fax +1 847 405 0164 Our expertise in microscopy is the basis for all our solutions for visualization, measurement and analysis of microstructures in life sciences and industry. With confocal laser technology and image analysis systems, we provide threedimensional viewing facilities and offer new solutions for cytogenetics, pathology and materials sciences. · Specimen Preparation · Medical Equipment Innovative technologies in our surgical microscopes offer new therapeutic approaches in microsurgery. @ www. histo-solutions. com Leica Microsystems Nussloch GmbH Heidelberger Strasse 17-19 D-69226 Nussloch Telephone: (06224) 143-0 Fax: (06224) 143 200 e-mail: histo_info@leica-microsystems. com www. leica-microsystems. com 09/2004 and representatives of Leica Microsystems in more than 100 countries. · Semiconductor Equipment Our automated, leading-edge measurement and inspection systems and our E-beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world. ©Leica Microsystems Nussloch GmbH, Heidelberger Str. [. . . ]